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| 产品名称： | clone 3.10 |
|---|---|
| 商品货号： | TS148932 |
| Designations： | clone 3.10 |
| GenBank Number： | M59911 |
| Species： | Homo sapiens, human |
| Depositors： | Q Yuan, ME Hemler |
| Vector： | Construct size (kb): 6.730000019073486 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: XbaI closer to SacI Vector end: XbaI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ITGA3 Gene name: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3 receptor) Contains complete coding sequence?: Y Tissue: endothelium cells and HepG2 cell line Type of DNA: cDNA Insert end: Modification: XbaI linker Insert end: Modification: SalI linker Insert size (kb): 3.47 Cross references: DNA Seq. Acc.: M59911 Nucleotides 1-3470 of the insert correspond to nucleotides 1-3470 of M59911. Insert lengths(kb): 3.47000002861023 Tissue: endothelium cells and HepG2 cell line Gene product: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3 receptor)(integrin, alpha 3 subunit) ITGA3 |
| Insert Size (kb)： | 3.470 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 3.0; HindIII--6.5; EcoRI--6.5; BamHI--3.1, 2.9, 0.5; NdeI--6.5. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--2.39; EcoRI--2.47; BamHI--2.7, 3.32. Constructed from clones 3.410, 3.285 and 3.520. |
| References： | Takada Y, et al. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J. Cell Biol. 115: 257-266, 1991. PubMed: 1655803 |