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| 产品名称： | pXC46 |
|---|---|
| 商品货号： | TS148949 |
| Designations： | pXC46 |
| Depositors： | TA Patterson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.78 Vector: pXC46 (plasmid) Promoters: Promoter for expression lambda PL Construction: pXC24, oligo cassette Marker(s):ampR Construct size (kb): 5.77 Features: initiation codon: ATG marker(s): ampR marker(s): tetR promoter for expression: lambda PL replicon: ROP copy number control replicon: pMB1 restriction site: 3 sites HindIII...BamHI restriction site: 5 SwaI...NsiI/NdeI sites ribosome-binding site: synthetic Shine-Dalgarno spacer sequence: ATTTAA transcription terminator: partially deleted lambda terminator coding sequence: 14-3-3 |
| Applications： | expression vector in another host, produces protein vector useful for cloning PCR products |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5 and 3 cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression. One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences. The insert is expressed as a Met-His fusion protein. Sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein, while sequences cloned into the SwaI site can be expressed as an unfused protein (must provide ATG in cloned insert). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761 |
| Shipped： | freeze-dried |