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| 产品名称： | pXPB Plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS149043 |
| Designations： | pXPB Plasmid in Escherichia coli |
| Depositors： | SK Nordeen |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.90
Vector: pXPB (plasmid)
Promoters: Promoter none
Construction: pSV232AL-Adelta5 derivative
Marker(s):ampR
Construct size (kb): 5.90
Features: insert detection: luciferase marker(s): ampR promoter: none replicon: pMB1 terminator: none enhancer: none |
| Applications： | xa0promoter-cloning vector
xa0shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; consistent with deposited DNA. This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509 de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727 Steven K Nordeen, personal communication |
| Shipped： | freeze-dried |