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微信小章| 产品名称： | pNB-1 pNb-1 |
|---|---|
| 商品货号： | TS149085 |
| Designations： | pNB-1 pNb-1 |
| GenBank Number： | K00057 |
| Species： | Homo sapiens, human |
| Depositors： | J Bishop |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 1.0 Tissue: Kelly neuroblastoma Gene product: avian myelocytomatosis viral (v-myc) related oncogene, neuroblastoma derived MYCN Alleles: C1, C1, C2, C2, C3, C3 |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--5.3; BamHI/EcoRI--4.3, 1.0; XhoI--5.5; PstI--3.7, 0.9, 0.8; EcoRI--5.5; XhoI--5.5. Includes part of intron 1 and exon 2; from approx 1188-2186 on Fig. 2, includes Pst I (2), XhoI, SmaI (2), SstI sites. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Stanton LW, et al. Nucleotide sequence of the human n-myc gene. Proc. Natl. Acad. Sci. USA 83: 1772-1776, 1986. PubMed: 2869488 Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081 Schwab M, et al. Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour. Nature 305: 245-248, 1983. PubMed: 6888561 Barbara Weiffenbach, personal communication |