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| 产品名称： | lambdaSP14 |
|---|---|
| 商品货号： | TS149160 |
| Designations： | lambdaSP14 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc |
| Vector： | Construct size (kb): 44.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 15.19999980926514 Tissue: T-lymphocyte SP-F7 cell line Gene product: T cell receptor, gamma, variable region V3 TCRGJP Alleles: A2, A1 |
| Insert Size (kb)： | 15.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--18.0, .5, 7.0, 6.2, 5.0; BamHI--20.0, 12.0, 9.0, 3.6; EcoRI--21.0, 12.0, 5.8, 2.3, 1.9, 1.3; XbaI--20.0, 15.0, 9.5; PvuI--22.0, 15.0, 7.5; SacI--19.0 9.5, 6.5, 4.2, 4.0. The insert contains the following restriction sites (approximate kb from the5 end): EcoRI--1.4, 3.2, 9.1, 10.7; 11.9; 13.8; HindIII--1.3, 5.8, 6.4, 8.3, 9.9, 12.1, 14.0; KpnI--3.4, 3.9, 8.1. Contains a non-functional V9-JP rearrangement generating a frameshift in the N diversity region. Also contains germline V2 and an unusual reqrrangement abutting V3 to J1 recombination signals in an inverted orientation. lambdaSP14 (TS149160, 65837) and lambdaSP19 (ATCC 65838, 65839) are end clones from the same inversion (47 kb) event. |
| References： | Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738 |