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| 产品名称： | pMMB277 |
|---|---|
| 商品货号： | TS149236 |
| Designations： | pMMB277 |
| Depositors： | VM Morales |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.2370004653930660 Vector: pMMB277 (plasmid) Promoters: Promoter tac Construction: pMMB206, pMMB311 Marker(s):cmlR Construct size (kb): 9.237000465393066 Features: insert detection: lacZ marker(s): cmlR promoter: tac replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8. Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter). |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347 |
| Shipped： | freeze-dried |