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| 产品名称： | pSNAG |
|---|---|
| 商品货号： | TS149240 |
| Designations： | pSNAG |
| Depositors： | C Hovens |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1960000991821290 Vector: pSNAG (phagemid) Promoters: Promoter CMV Construction: pSCTEV, pSuperVSV Marker(s):ampR Construct size (kb): 5.196000099182129 Features: marker(s): ampR other: Gal4p DNA-binding domain (DBD) promoter: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: M13 replicon: SV40 ori replicon: pMB1 replicon: polyoma MCS: SfuI...XbaI terminator: SV40 epitope tag: VSV-G |
| Applications： | expression vector vector permitting construction of fusion proteins vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2. The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205 |
| Related Products： | component of:ATCC 87677 |
| Shipped： | freeze-dried |