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| 产品名称： | pCN41 |
|---|---|
| 商品货号： | TS149288 |
| Designations： | pCN41 |
| Depositors： | R Diaz Orejas |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5000000000000000 Vector: pCN41 (plasmid) Construction: pCN40 Marker(s):strR Construct size (kb): 4.5 Features: marker(s): strR replicon: repA |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. General purpose vector. Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating. |
| Media： | ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Growth Conditions： | Temperature: 37.0°C |
| References： | Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095 Ramon Diaz Orejas, personal communication |
| Shipped： | freeze-dried |