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| 产品名称： | 5HT1Dalpha |
|---|---|
| 商品货号： | TS149294 |
| Designations： | 5HT1Dalpha |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D alpha |
| Vector： | Construct size (kb): 9.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: p3CLHygro Intact vector size: 8.300 Type of vector: plasmid Vector end: SalI Vector end: SalI Cloning sites: HindIII SalI Polylinker sites: HindIII SalI Construction: p3CL, pHEBo (hygR) Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, hygR promoter: CMV replicon: pMB1, SV40 terminator: bGH polyadenylation Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HTR1DA* Gene name: 5-hydroxytryptamine (serotonin) receptor 1D alpha Contains complete coding sequence?: Y Type of DNA: genomic Insert end: SalI Insert end: SalI Insert size (kb): 1.1 Cross references: Insert lengths(kb): 1.100000023841858 Gene product: 5-hydroxytryptamine (serotonin) receptor 1D alpha HTR1DA* Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D alpha |
| Insert Size (kb)： | 1.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 0.92; BglII--7.8, 1.2, 0.48; HindIII--8.3, 0.92, 0.29; SalI--8.3, 1.2; XhoI--5.2, 4.3; EcoRI--6.0, 2.4, 1.0. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.09, 1.00; BglII--0.11, 0.58; HindIII--0.84, 1.10; XhoI--0.88. Contains the complete coding sequence with only a few extra nucleotides. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - hygR - tk polyadenylation - TaqI - SV40 polyadenylation - EcoRI - CMV promoter >- HindIII - SalI - insert >- SalI - bGH polyadenylation - PvuI. |
| References： | Sarah A Veldman, personal communication |