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| 产品名称： | YIp29 pRB20 |
|---|---|
| 商品货号： | TS149303 |
| Designations： | YIp29 pRB20 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.8000001907348630 Vector: YIp29 (plasmid) Construction: pBR322, Saccharomyces cerevisiae Marker(s):LEU2,URA3,ampR Construct size (kb): 7.800000190734863 Features: marker(s): ampR, LEU2, URA3 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--4.7, 3.2; BamHI--7.8. A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3 untranslated regions of URA3(SmaI) and LEU2(SalI). Orientations are such that transcription from both URA3 and LEU2 is clockwise. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Shipped： | freeze-dried |