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| 产品名称： | human CAV cDNA |
|---|---|
| 商品货号： | TS149311 |
| Designations： | human CAV cDNA |
| GenBank Number： | L19297 |
| Species： | Homo sapiens, human |
| Depositors： | WS Sly |
| Vector： | Construct size (kb): 4.079999923706055 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: XhoI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CA5 Genomic copy number: unique Gene name: carbonic anhydrase V Contains complete coding sequence?: Y Chromosome: 16 Tissue: liver Type of DNA: cDNA Insert 5 end: Modification: EcoRI linker Insert 3 end: Modification: XhoI linker Insert size (kb): 1.123 Cross references: DNA Seq. Acc.: L19297 Nucleotides 1-1123 of the insert correspond to nucleotides 1-1123 of L19297. Insert lengths(kb): 1.123000025749207 Tissue: liver Gene product: carbonic anhydrase V CA5 |
| Insert Size (kb)： | 1.123 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BamHI/XhoI--3.0, 1.1; BglI--1.9, 1.3, 0.9; EcoRI--3.6, 0.52; PstI--3.6, 0.4. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.29; BglI--0.38; EcoRI--0.53; PstI--0.63. |
| References： | Nagao Y, et al. Human mitochondrial carbonic anhydrase: cDNA cloning, expression, subcellular localization, and mapping to chromosome 16. Proc. Natl. Acad. Sci. USA 90: 7623-7627, 1993. PubMed: 8356065 |