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微信小章| 产品名称： | p3 |
|---|---|
| 商品货号： | TS149341 |
| Designations： | p3 |
| GenBank Number： | M73969 |
| Species： | Homo sapiens, human |
| Depositors： | PM Murphy |
| Applications： | A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe. |
| Vector： | Construct size (kb): 4.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: XhoI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IL8RB Gene name: interleukin 8 receptor, beta Contains complete coding sequence?: Y Chromosome: 2; Localization: 2 q35 Tissue: HL-60 cell line Disease: promyelocytic leukemia Type of DNA: cDNA Insert 5 end: Modification: EcoRI linker Insert 3 end: Modification: XhoI linker Insert size (kb): 1.7 Cross references: DNA Seq. Acc.: M73969 Nucleotides 165-1675 of the insert correspond to nucleotides 1-1510 of M73969. Insert lengths(kb): 1.700000047683716 Tissue: HL-60 cell line promyelocytic leukemia Disease: promyelocytic leukemia Gene product: interleukin 8 receptor, beta IL8RB |
| Insert Size (kb)： | 1.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.7; XhoI--4.7; EcoRI/XhoI--2.9, 1.8; PstI--4.7; BglII/HindIII--3.5, 1.2. Includes 156 nt in addition to the 5 untranslated region reported in GenBank M73969. Nucleotides 1-9 of the insert are linker. A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe. |
| References： | Murphy PM, Tiffany HL. Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253: 1280-1283, 1991. PubMed: 1891716 Ahuja SK, et al. Molecular evolution of the human interleukin-8 receptor gene cluster. Nat. Genet. 2: 31-36, 1992. PubMed: 1303245 Ahuja SK, Murphy PM. Molecular piracy of mammalian interleukin-8 receptor type B by Herpesvirus Saimiri. J. Biol. Chem. 268: 20691-20694, 1993. PubMed: 8407886 Ahuja SK, et al. Comparison of the genomic organization and promoter function for human interleukin-8 receptors A and B. J. Biol. Chem. 269: 26381-26389, 1994. PubMed: 7929358 Ake Lernmark, personal communication |