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| 产品名称： | pPR5 |
|---|---|
| 商品货号： | TS149413 |
| Designations： | pPR5 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | OmniGene, Inc., KM Overbye, OmniGene, Inc. |
| Applications： | produces protein alkaline phosphatase |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: alkaline phosphatase phoA Target Gene: alkaline phosphatase |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 2.7; SmaI--5.9; BamHI--5.7, 0.2; XhoI--5.9. Construct permitting expression and secretion of alkaline phosphatase using the chitinase promoter and signal sequences. The insert contains the complete coding sequence for phoA, as well as the induction signals, ribosome-binding site and the signal sequence for S. plicatus chitinase-56 (also called chitinase-63). The preferred cleavage site is after Ala30. A HindIII digest yields a 3.2 kb fragment, containing approximately 250 bp of Streptomyces DNA (the chitinase regulatory sequences), and coding for a chitinase-phoA fusion protein. The phoA sequences can be removed vy a SmaI/XhoI digest and replaced with other sequences to be expressed and secreted. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Overbye KM, et al. DNA encoding a Streptomyces endochitinase 56 signal peptide. US Patent 5,188,961 dated Feb 23 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |