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| 产品名称： | 45.1 DObeta 163 45.1 DOBeta 163 |
|---|---|
| 商品货号： | TS149478 |
| Designations： | 45.1 DObeta 163 45.1 DOBeta 163 |
| GenBank Number： | X03068 |
| Species： | Homo sapiens, human |
| Depositors： | EO Long |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.398000001907349 Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2) Gene product: major histocompatibility complex, class II, DO beta HLA-DOB |
| Insert Size (kb)： | 1.398 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.45; PstI--2.0, 1.4, 1.28; HindIII--2.6, 1.3. Insert includes 56 bp 5 untranslated, and open reading frame of 273 codons, and 447 bp 3 untranslated sequence. BamHI site at 3 end is within SV40 sequence (coordinates 0.715-0.710). Insert cut out with BamHI will include 35 bp of SV40. Cross-hybridizes with rabbit sequences. |
| References： | Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758 Kulaga H, et al. Expression patterns of MHC class II genes in rabbit tissues indicate close homology to human counterparts. J. Immunol. 139: 587-592, 1987. PubMed: 3474276 |