| 产品名称: | HuHsp90-#2 |
|---|---|
| 商品货号: | TS149479 |
| Designations: | HuHsp90-#2 |
| GenBank Number: | M16660 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 5.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS- Intact vector size: 2.964 Type of vector: phagemid Vector end: SalI Vector end: SalI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HSPCB Other gene symbols: HSPC2 Gene name: heat shock 90kD protein 1, beta Contains complete coding sequence?: Y Localization: 6 p12 Tissue: fetal liver Type of DNA: cDNA Insert end: SalI Insert end: SalI Insert size (kb): 2.739 Cross references: DNA Seq. Acc.: M16660 Nucleotides 13-2524 of the insert correspond to nucleotides 32-2543 of M16660. Insert lengths(kb): 2.739000082015991 Tissue: fetal liver Gene product: heat shock 90kD protein 1, beta HSPC2 |
| Insert Size (kb): | 2.739 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--4.9, 0.7; HindIII--4.4, 1.4; KpnI--5.0, 0.64; PvuII--2.5, 1.7, 1.5; SalI--3.0, 2.7. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.08; BglI--0.11; EcoRI--0.97, 1.05; HindIII--1.38; KpnI--0.60; PstI--2.17, 2.30; PvuII--1.16; SacI--1.30; XhoI--2.20, 2.31. The SalI insert contains the complete coding sequence and a poly(A) tail followed by a cloning artifact of approximately 180 bp. The genomic sequence is available in GenBank/EMBL/DDBJ accession J04988. |
| References: | Rebbe NF, et al. Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene. J. Biol. Chem. 264: 15006-15011, 1989. PubMed: 2768249 Rebbe NF, et al. Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family. Gene 53: 235-245, 1987. PubMed: 3301534 Neil F Rebbe, personal communication |