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| 产品名称： | cHuVim1 cHuVIM1 |
|---|---|
| 商品货号： | TS149488 |
| Designations： | cHuVim1 cHuVIM1 |
| Species： | Homo sapiens, human |
| Depositors： | D Paulin |
| Applications： | Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.100000023841858 Tissue: fibroblast Gene product: vimentin VIM Alleles: A1, A2 |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains the following sites separated by (bp) (approx): EcoRI- 130- MspI- 120- MspI- 35- HincII- 95- PstI- 115- PstI- 100- MspI- 140- PstI- 140- MspI- 170- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; HindIII--4.0; PstI--3.1, 0.46, 0.29; BamHI--3.8; SalI--4.0. Clone starts at nt 331 (corresponding to amino acid 111) after the ATG and extends to the stop codon. Includes part of exon 1 through exon 9. Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines. |
| References： | Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665 |