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微信小章| 产品名称： | Chlamydia trachomatis Serovar G |
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| 商品货号： | TS149513 |
| Classification： | Chlamydiaceae |
| Deposited As： | Trachoma serotype g |
| Agent： | Chlamydia trachomatis Serovar G |
| Strain： | UW-57/Cx |
| Applications： | Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 36°C for 72 hours. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa |
| Recommended Host： | McCoy cells (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
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| Name of Depositor： | SP Wang |
| Source： | Human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971 |
| References： | Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875 |