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| 产品名称： | pGEM2MCSF10 CMCC 2760 |
|---|---|
| 商品货号： | TS149647 |
| Designations： | pGEM2MCSF10 CMCC 2760 |
| Species： | Mus musculus, mouse |
| Depositors： | JC Hunter-Cevera |
| Vector： | Construct size (kb): 6.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.0 Gene product: colony stimulating factor, macrophage Csfm |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites separated by (bp)(approximate): EcoRI- 310- PstI- 630- AvaI- 136- HindIII- 533- BamHI- 1390- HindIII- 932- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.85, 2.9; HindIII--3.8, 1.95, 0.95; PstI--3.65, 3.1; BamHI--4.35, 2.35; XbaI--6.8. The insert is oriented in the sense direction relative to the SP6 promoter, such that RNA transcription will give translatable mRNA. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Ladner MB, et al. cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. Proc. Natl. Acad. Sci. USA 85: 6706-6710, 1988. PubMed: 2457916 Jennie C Hunter-Cevera, personal communication |