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| 产品名称： | Chlamydophila pneumoniae |
|---|---|
| 商品货号： | TS149759 |
| Classification： | Chlamydiaceae, Chlamydophila |
| Deposited As： | Chlamydia pneumoniae Grayston et al. |
| Agent： | Chlamydophila pneumoniae |
| Strain： | CM-1 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | This isolate was identified as C. pneumoniae by culture characteristics, morphology of inclusion bodies, and reaction with monoclonal antibodies by depositor. Item isolated on cycloheximide-treated HeLa 229 cells, and found to be serologically identical to the reference strain TW183 by micro-immunofluorescent test. |
| Effect on Host： | Intracellular glycogen negative inclusion bodies visualized by fluorescent staining |
| Recommended Host： | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-95% confluent. Vortex material with 1mm glass beads to disrupt cells. Remove cell growth medium and inoculate with disrupted material. Centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium. Incubation: 3 days |
| Name of Depositor： | KH Wong, Centers for Disease Control and Prevention |
| Special Collection： | NCRR Contract |
| Source： | Sputum of pneumonia patient, Georgia |
| References： | Black CM, et al. Antigenic variation among strains of Chlamydia pneumoniae. J. Clin. Microbiol. 29: 1312-1316, 1991. PubMed: 1885727 |
| Cross References： | Nucleotide (GenBank) : U44033 Chlamydia pneumoniae putative ribonuclease P RNA gene. |