宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pBShUBC9(S)

货号 TS149773
中文名称 null
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产品名称: pBShUBC9(S)
商品货号: TS149773
Designations: pBShUBC9(S)
GenBank Number:

U66818

Species: Homo sapiens, human
Depositors: C Wang
Applications:
contains sequence ubiquitin-conjugating enzyme E2I
Vector:
Construct size (kb): 4.050000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 1.100000023841858
Tissue: placenta
Gene product: ubiquitin-conjugating enzyme E2I UBC9
Target Gene: ubiquitin-conjugating enzyme E2I
Insert Size (kb): 1.100
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.1; PvuII--2.6, 1.0, 0.45; XbaI--3.1, 0.88.
The insert contains the following restriction sites (approximate kb from the 5 end): NotI--0.06; AvaI--0.07; StuI--0.48; PvuII--0.94; XbaI--0.98.
ATCC 99599 and TS149773 are the same for the initial 15 bp upstream of the initiation codon, the CDS, and the 3 UTR. The clones differ at the 5 end of the 5 UTR. ATCC 99599 contains a 791 bp sequence while TS149773 has a different 72 bp sequence.
References:

Wang ZY, et al. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. PubMed: 8798754