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| 产品名称： | pKSM718 |
|---|---|
| 商品货号： | TS149775 |
| Designations： | pKSM718 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5840001106262210 Vector: pKSM718 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pUC18 Marker(s):ampR Construct size (kb): 3.584000110626221 Features: insert detection: lacZalpha marker(s): ampR promoter for expression: lacUV5 promoter for in vitro transcription: T7lac replicon: fd replicon: pMB1 MCS: DsaI...EcoRI transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--3.29; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.04; PvuII--0.48. Differs from pKSM717 (ATCC 87162) only in the orientation of the ampicillin resistance gene. Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |