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| 产品名称： | YEpFAT3 |
|---|---|
| 商品货号： | TS149816 |
| Designations： | YEpFAT3 |
| Depositors： | K Runge |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli MC1066 |
| Vector Information： | Size (kb): 7.5359997749328610 DESCRIPTION OF VECTOR: Intact vector size: 7.536 Type of vector: plasmid Cloning sites: NotI SacI SmaI BamHI XbaI Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI Other unique sites: NdeI HindIII Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7, ->, 860-879 MCS: NotI...XbaI, ->, 908-942 replicon: 2 micron/LEU2-d, 941-3794 marker(s): LEU2-d, ->, 2298-3392 marker(s): URA4 from S. pombe, restriction site: HindIII, 5265-5265 promoter for in vitro transcription: T3, promoter: lac, replicon: pMB1, 5718-5718 marker(s): ampR, Vector: YEpFAT3 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):ampR,LEU2-d,ura4+ Construct size (kb): 7.535999774932861 Features: marker(s): LEU2-d marker(s): URA4 from S. pombe marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron/LEU2-d replicon: pMB1 MCS: NotI...XbaI restriction site: HindIII |
| Applications： | M9 + leucine + tryptophan culture media recommended for DNA purification. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.5; SmaI--7.5; EcoRI--5.4, 2.1. M9 + leucine + tryptophan culture media recommended for DNA purification. One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |