| 产品名称: | pB1.4 hum pB1.4.hum |
|---|---|
| 商品货号: | TS149839 |
| Designations: | pB1.4 hum pB1.4.hum |
| GenBank Number: | X52882 |
| Species: | Homo sapiens, human |
| Depositors: | KR Willison |
| Vector: | Construct size (kb): 3.299999952316284 |
| Insert: | DNA: cDNA Insert lengths(kb): 0.5799999833106995 Gene product: t-complex 1(T-complex protein 1) TCP1 Alleles: A1, A2 |
| Insert Size (kb): | 0.580 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 0.6; BamHI--3.3; PstI--3.3; HindIII--3.3. There are 4 TCP1-related sequences/haploid genome--1 expressed and 3 pseudogenes. One pseudogene maps to 7p14-cen, another may to 5. The expressed sequence shows most homology to mouse; all human sequences are more similar than to mouse. Insert encodes 3 sequences. Shows about 90% homology with mouse sequences and determines an amino acid sequence showing about 98% homology with mouse protein. The mouse and human TCP1 ORFs both encode polypeptides of 556 amino acids which are 95.5% homologous. Detects mRNAs of 1.7 kb (major) and 2.4 kb (minor). Differences are apparently due to polyadenylation. |
| References: | Fonatsch C, et al. Assignment of the TCP1 locus to the long arm of human chromosome 6 by in situ hybridization. Cytogenet. Cell Genet. 45: 109-112, 1987. PubMed: 3476253 Willison K, et al. Chromosomal assignment of TCP-1 the human homologue of a mouse T-complex locus. Cytogenet. Cell Genet. 40: 779-780, 1985. Kirchhoff C, Willison K. Nucleotide and amino-acid sequence of human testis-derived TCP1. Nucleic Acids Res. 18: 4247, 1990. PubMed: 2377466 Willison K, et al. The human homologue of the mouse T-complex gene, TCP1, is located on chromosome 6 but is not near the HLA region. EMBO J. 6: 1967-1974, 1987. PubMed: 3653076 |