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| 产品名称： | AR4c |
|---|---|
| 商品货号： | TS149870 |
| Designations： | AR4c |
| GenBank Number： | X83543 |
| Species： | Homo sapiens, human |
| Depositors： | MV Schiaffino |
| Applications： | contains sequence apical protein, Xenopus laevis-like |
| Vector： | Construct size (kb): 6.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: APXL Gene name: apical protein, Xenopus laevis-like Contains complete coding sequence?: N Tissue: retina, adult Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 3.647 Cross references: DNA Seq. Acc.: X83543 Nucleotides 1-3637 of the insert correspond to nucleotides 3808-7445 of X83543. Insert lengths(kb): 3.647000074386597 Tissue: retina, adult Gene product: apical protein, Xenopus laevis-like APXL Target Gene: apical protein, Xenopus laevis-like |
| Insert Size (kb)： | 3.647 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.0; BamHI--6.3, 0.4; HindIII-- 6.0, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.35; BglI--0.05, 0.15, 0.55, 2.85, 2.9; HindIII--3.05; NcoI--2.15; SacI--0.65, 1.0, 1.25; SmaI--0.85; PstI--0.6, 0.7; PvuII--1.15, 3.05, 3.35, 3.5; XhoI--3.3. The insert contains a portion of the coding sequence and 2509 bp of 3 untranslated sequence, overlapping AR24 (ATCC 99512) at the 5 end. The coding region of GenBank X83543 corresponds to nt 91-4941. |
| References： | Schiaffino MV, et al. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Mol. Genet. 4: 373-382, 1995. PubMed: 7795590 |