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| 产品名称： | pWE3 |
|---|---|
| 商品货号： | TS149915 |
| Designations： | pWE3 |
| Depositors： | M Cockett |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5019998550415040 Vector: pWE3 (phagemid) Promoters: Promoter for in vitro transcription SP6 Marker(s):ampR,purR Construct size (kb): 6.501999855041504 Features: marker(s): ampR marker(s): puromycin resistance promoter for expression: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: SV40 replicon: f1 replicon: pMB1 MCS: HindIII...ApaI terminator: SV40 polyadenylation enhancer: SV40 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.5; SalI--3.6, 2.9. Having identical cloning sites also facilitates shuttling cDNAs from one vector to another. One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678). Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206 |
| Related Products： | component of:ATCC 87678 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried xa0E. coli containing the plasmid |