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| 产品名称： | YCpIF17 |
|---|---|
| 商品货号： | TS149919 |
| Designations： | YCpIF17 |
| Depositors： | RW Davis, PK Foreman |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 5.30
Vector: YCpIF17 (plasmid)
Promoters: Promoter for expression GAL1
Construction: YCpIF15
Marker(s): TRP1,ampR
Construct size (kb): 5.30
Features: marker(s): TRP1 marker(s): ampR promoter for expression: GAL1 replicon: ARS1 replicon: pMB1 MCS: SacI...ClaI centromere: CEN4 epitope tag: hemaglutinin (HA), influenza virus |
| Applications： | YX-type (expression) shuttle vector encodes an epitope tag for protein isolation or monitoring |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 1.8; BamHI--5.3; EcoRI--5.3. Yeast expression vector allowing production of a protein tagged at the N-terminal end with a hemaglutinin epitope. Vectors YCpIF15, YCpIF16 and YCpIF17 (ATCC 87090-87092) differ only in the reading frame of the multiple cloning site. The encoded nine aa of the influenza virus hemaglutinin epitope are recognized by the monoclonal antibody 12CA5, allowing detection and affinity purification of the recombinant protein. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907 |
| Related Products： | component of:ATCC 87134 |
| Shipped： | freeze-dried |