微信小陈
微信小章
产品简介
购买须知
| 产品名称： | GRF-1(1-835) UTR-02221, WI-9140 |
|---|---|
| 商品货号： | TS149965 |
| Designations： | GRF-1(1-835) UTR-02221, WI-9140 |
| GenBank Number： | M73077 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein glucocorticoid receptor DNA binding factor 1 |
| Vector： | Construct size (kb): 10.60000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRLF1 Gene name: glucocorticoid receptor DNA binding factor 1 Contains complete coding sequence?: Y Chromosome: 19; Localization: 19, 341.990000 cR Tissue: breast adenocarcinoma MCF7 cell line Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.9 Cross references: DNA Seq. Acc.: M73077 Insert lengths(kb): 4.900000095367432 Tissue: breast adenocarcinoma MCF7 cell line Gene product: glucocorticoid receptor DNA binding factor 1 GRLF1 Target Gene: glucocorticoid receptor DNA binding factor 1 |
| Insert Size (kb)： | 4.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.0, 4.6; EcoRI--5.4, 5.0; EcoRV--5.4, 2.9, 2.3; PvuII--10.5; SacI--6.0, 4.7; XbaI--4.7, 3.1, 2.4. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--1.47; BglII--1.67; EcoRV--1.84; NcoI--0.03, 2.14; NdeI--1.73; PstI--0.10, 1.82, 2.76; PvuII--0.55; SacI--0.72; SmaI--1.79; XbaI--2.30. Contains the complete coding sequence (nt 34-2541) plus 1.5 kb 3 untranslated. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. |
| References： | LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621 |