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| 产品名称： | pCLL9 |
|---|---|
| 商品货号： | TS149972 |
| Designations： | pCLL9 |
| Depositors： | American Cyanamid Co., JS Feitelson, American Cyanamid Co. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.9099998474121090 Vector: pCLL9 (plasmid) Construction: pCLL7, pIJ702 Marker(s):ampR,tsrR Construct size (kb): 9.90999984741211 Features: insert detection: mel marker(s): ampR marker(s): tsrR replicon: pAkij1 |
| Applications： | shuttle vector vector permitting visual detection of recombinants tyrosinase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--10.2; BglII--10.2; PstI--10.2. Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli. Cloning into the BglII, SphI, or SstI sites inactivates the tyrosinase gene. The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991 Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302 |
| Shipped： | freeze-dried |