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| 产品名称： | pHS24 |
|---|---|
| 商品货号： | TS149973 |
| Designations： | pHS24 |
| Depositors： | HG Sass |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 12.0000000000000000 Vector: pHS24 (plasmid) Promoters: Promoter hsp82 Construction: pHS17 Marker(s):G418R,ampR,Adh+ Construct size (kb): 12.0 Features: marker(s): ampR, G418R, Adh+ promoter: hsp82 replicon: pMB1 terminator: hsp82 |
| Applications： | contains sequence alcohol dehydrogenase integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--12.5; XbaI--11.0, 0.72, 0.50, 0.37; BamHI/KpnI--7.6, 3.8, 1.3. Expression is constitutive, but can be increased with heat. Selection of transgenic Drosophila by G418 has been done with embryos, larvae, and adults. Adults can be reselected by 6% ethanol. Can be used in Drosophila spp. cell culture transfection assays, and may function in other animal and plant systems because the hsp82 promoter is highly conserved. P-transposable (integrating) shuttle vector. Contains hsp82-neo fusion gene which encodes the first 20 aa of D. pseudoobscura hsp82, 5 codons in a linker, fused to the fifth codon of bacterial neomycin phosphotransferase from Tn5, and followed by polyadenylation signals from D. melanogaster hsp82. Also contains a complete alcohol dehydrogenase coding region from D. melanogaster (3.2 kb XbaI fragment) which includes 0.66 kb of 5 and 0.64 kb of 3 DNA, and has all the cis-acting sequences necessary for expression. The order of the major components of this construct are: 3 P-element, 5-Adh-3, 5-hsp82-neo fusion-hsp82 polyadenylation signal-3, KpnI site, and 5 P-element. Differs from pHS22 (ATCC 37840) by the removal of hsp82 upstream sequences from approximately -5000 to -868 (where the transcription start point is +1). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sass H. P-transposable vectors expressing a constitutive and thermoinducible hsp82-neo fusion gene for Drosophila germline transformation and tissue-culture transfection. Gene 89: 179-186, 1990. PubMed: 2165019 |
| Shipped： | frozen |
| Shipping Information： | Distributed: frozen |