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| 产品名称： | pRB394 |
|---|---|
| 商品货号： | TS149983 |
| Designations： | pRB394 |
| Depositors： | R Bruckner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5000000000000000 Vector: pRB394 (plasmid) Construction: pUB110, pBR322, cat Marker(s):ampR,bleR,neoR Construct size (kb): 6.5 Features: insert detection: CAT marker(s): ampR, neoR, bleR replicon: pUB110, pMB1 terminator: rrnB, to phage lambd |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; BamHI--6.7; BglI/BglII--3.5, 3.2. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Promoter-cloning shuttle vector using the expression of chloramphenicol acetyltransferase (CAT) as the reporter. Also useful for construction of fusion proteins. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. The cat gene was derived from pUB112 by deletion of the promoter and the regulatory inverted repeat, resulting in constitutive cat gene expression when provided with an appropriate upstream promoter. The order of the major features in the plasmid is: To terminator - HindIII/MCS/EcoRI - cat - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028 |
| Shipped： | freeze-dried |