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微信小章| 产品名称： | Gallid herpesvirus 2 |
|---|---|
| 商品货号： | TS150085 |
| Classification： | Herpesviridae, Mardivirus |
| Deposited As： | Mareks disease virus |
| Agent： | Gallid herpesvirus 2 |
| Strain： | GA-5 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | chicken tissue with Mareks disease |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Comments： | Infected CK cell suspension with 7% DMSO + Penicillin/Streptomycin + Fungizone®. Similar to JM strain. Dilution of infected chicken kidney cell suspension with medium 2-3 times the volume after thawing will enhance virus recovery. |
| Effect on Host： | CPE, focal lesions |
| Recommended Host： | chicken kidney cells |
| Growth Conditions： | Temperature: 38.5°C Incubation: 5-7 days |
| Name of Depositor： | BW Calnek, KA Schat, Cornell University New York State College of Veterinary Medicine |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Chicken tissue with Marek?s disease, Georgia, 1964 |
| References： | Calnek BW, Hitchner SB. Highly potent, viable and stable cell-free virus preparations from cells infected with cell-associated viruses and method for obtaining the same. US Patent 3,783,098 dated Jan 1 1974 Eidson CS, Schmittle SC. Studies on acute Mareks disease. I. Characteristics of isolate GA in chickens. Avian Dis. 12: 467-476, 1968. PubMed: 4301071 Reddy SM, et al. Development of a quantitative-competitive polymerase chain reaction assay for serotype 1 Mareks disease virus. Avian Dis. 44: 770-775, 2000. PubMed: 11195630. Silva RF. Differentiation of pathogenic and non-pathogenic serotype 1 Mareks disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Dis. 36: 521-528, 1992. PubMed: 1329706 |
| Cross References： | Nucleotide (GenBank) : U63464 Gallid herpesvirus 2 DNA polymerase gene, partial cds. |