宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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ph1

货号 TS150173
中文名称 null
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产品简介
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产品名称: ph1
商品货号: TS150173
Designations: ph1
GenBank Number:

M77844

Species: Homo sapiens, human
Depositors: GF Saunders
Vector:
Construct size (kb): 5.599999904632568
Insert:
DNA: cDNA
Insert lengths(kb): 2.700000047683716
Tissue: eye, fetal
Gene product: paired box homeotic gene 6 (aniridia, keratitis)( paired box homeotic protein, aniridia locus, aniridia 2 (without Wilms tumor, genitourinary abnormalities, and mental retardation), aniridia 2 (without Wilms tumor, genitourinary abnormalities, and mental retardation), paired box homeotic gene aniridia) AN2
Insert Size (kb): 2.700
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.35; PstI--4.4, 1.35; PvuII--2.7, 2.1, 1.0; BglII--5.8; HindIII--5.8; EcoRI/PvuI--3.0, 1.4, 1.1, 0.2; EcoRI/PvuII--2.6, 1.4, 1.0, 0.55, 0.2.
Restriction digests of the clone give the following sizes (kb): NotI/SalI--3.0, 2.7.
The insert spans two adjacent genomic NotI fragments of 325 kb and 1400 kb. The 5 end is in the 325 kb NotI fragment.
Corresponds to the 3 portion of the reported sequence.
Overlaps ph12 (ATCC 65958, 65959).
One of the EcoRI sites on the insert ends was lost during cloning.
References:

Ton CC, et al. Positional cloning and characterization of a paired box- and homeobox-containing gene from the aniridia region. Cell 67: 1059-1074, 1991. PubMed: 1684738

Grady F Saunders, personal communication