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微信小章| 产品名称： | pDP34 |
|---|---|
| 商品货号： | TS150179 |
| Designations： | pDP34 |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy, homologous on X and Y DXYS1 Alleles: B1, B2, A1, A2, B1, B2, A1, A2 |
| Insert Size (kb)： | 2.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.25 kb triplet; HindIII--2.4 kb, 2.2 kb doublet; BamHI--6.6; PstI--2.2, 1.6, 1.5, 1.3. As there is only 1 BamHI site, there seem to be 2 inserts. pDP34 corresponds to probe 7 in Fig. 2. pDP34 shows linkage at a LOD score of 3.07 (theta=0) to cleft palate + ankyloglossia in a large Icelandic family. DXYS1 is linked to choroideremia with theta max = 0.02, LOD score = 10.25. This segment is deleted in XL-62 family. The insert detects a TaqI RFLP which maps to Xq21. The insert detects a MspI RFLP which maps to Yp. To avoid lane background, hybridize at high stringency (e.g., 47C in 50% formamide, 5X SSC; wash at 60C in 0.1X SSC, 0.1% SDS). |
| References： | Page DC, et al. Occurrence of a transposition from the X-chromosome long arm to the Y-chromosome short arm during human evolution. Nature 311: 119-123, 1984. PubMed: 6088994 Page D, et al. Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes. Proc. Natl. Acad. Sci. USA 79: 5352-5356, 1982. PubMed: 6291041 Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012 Schwartz CE, et al. Allan-Herndon syndrome. II. Linkage to DNA markers in Xq21. Am. J. Hum. Genet. 47: 454-458, 1990. PubMed: 2393020 Moore GE, et al. Linkage of an X-chromosome cleft palate gene. Nature 326: 91-92, 1987. PubMed: 2881212 Lesko JG, et al. Multipoint linkage analysis of loci in the proximal long arm of the human X chromosome: Application to mapping the choroideremia locus. Am. J. Hum. Genet. 40: 303-311, 1987. PubMed: 2883887 David C Page, personal communication |