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微信小章| 产品名称： | AM6 EC-AM6, pAM6 in E. coli |
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| 商品货号： | TS150234 |
| Designations： | AM6 EC-AM6, pAM6 in E. coli |
| Species： | Hepatitis B virus, Hepatitis B virus |
| Depositors： | WH Hoyer |
| Vector： | Name of construct: AM6
Other name(s): pAM6
Construct size (kb): 7.5
Markers: ampR
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI, ClaI, HindIII, EcoRV, BamHI, SphI, SalI, XmaIII, NruI, BspMI, BsmI, StyI, AvaI, BalI, BspMII, PvuII, Tth111I, NdeI, AflIII, PpaI, PstI, PvuI, ScaI, SspI, AatII
Construction: pBR313
Host Range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross ref: DNA seq. Acc.: J01749
Transformant name: EC-AM6 |
| Insert： | Genome: Hepatitis B virus
Contains complere coding sequence?: U
Strain: serotype adw
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.2 |
| Insert Size (kb)： | 3.20 |
| Putative ID： | Hepatitis B virus |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Purified plasmid DNA: 45020D |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Glycerol stock of E. coli containing the plasmid |
| Comments： | The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles and cloned into the pBR322 vector. The complete viral genome was linearized with BamHI and inserted into pBR322. A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product. The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg. This AM6 clone is available as ATCC 39630, 40101, 45020 and 45020D. ATCC 40101 and 45020D are the purified plasmid DNAs and ATCC 39630 and 45020 are the plasmids in E. coli. Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.85, 1.35; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96. |
| Classification： | Hepadnaviridae, Orthohepadnavirus |
| References： | Moriarty AM, et al. Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc. Natl. Acad. Sci. USA 78: 2606-2610, 1981. PubMed: 6264484 |