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| 产品名称： | p4 |
|---|---|
| 商品货号： | TS150302 |
| Designations： | p4 |
| GenBank Number： | L10918 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: XhoI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CMKBR1 Gene name: chemokine (C-C) receptor 1 Contains complete coding sequence?: Y Chromosome: 3; Localization: 3 p21 Tissue: HL-60 cell line Disease: promyelocytic leukemia Type of DNA: cDNA Insert 5 end: Modification: EcoRI linker Insert 3 end: Modification: XhoI linker Insert size (kb): 2.156 Cross references: DNA Seq. Acc.: L10918 Nucleotides 1-2156 of the insert correspond to nucleotides 1-2156 of L10918. Insert lengths(kb): 2.155999898910523 Tissue: HL-60 cell line promyelocytic leukemia Disease: promyelocytic leukemia Gene product: chemokine (C-C) receptor 1( chemokine (C-C) receptor 1, RANTES receptor) CMKBR1 |
| Insert Size (kb)： | 2.156 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 2.2; EcoRI--4.6, 0.6; XhoI--5.2; EcoRI/XhoI--3.0, 1.6, 0.6; PstI--4.6, 0.6. The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.45; EcoRI--0.59; PstI--0.61. |
| References： | Gao JL, et al. Structure and functional expression of the human macrophage inflammatory protein 1alpha/RANTES receptor. J. Exp. Med. 177: 1421-1427, 1993. PubMed: 7683036 |