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| 产品名称： | pRCN005 pRC2 |
|---|---|
| 商品货号： | TS150310 |
| Designations： | pRCN005 pRC2 |
| GenBank Number： | X08021 |
| Species： | Red clover necrotic mosaic virus |
| Depositors： | Z Xiong |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.435999989509583 Gene product: 35 kDa protein |
| Insert Size (kb)： | 1.436 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (nucleotide positions according to the published sequence): BglII--911; XbaI--1029. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; PstI--2.7, 1.5; consistent with deposited DNA. This clone includes the final nucleotide positions 15-1451. The single large open reading frame extends from 80 to 1031. There are other in frame AUG codons at positions 309, 312, 377, 428, and 688. The insert is oriented such that linearizing with EcoRI and transcription with SP6 RNA polymerase gives the positive polarity (sense) transcript whereas linearizing with HindIII and transcription with T7 RNA polymerase gives the antisense strand. |
| Classification： | Dianthovirus |
| References： | Lommel SA, et al. The nucleotide sequence and gene organization of red clover necrotic mosaic virus RNA-2. Nucleic Acids Res. 16: 8587-8602, 1988. PubMed: 3047682 |