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| 产品名称： | 2Q5 |
|---|---|
| 商品货号： | TS150336 |
| Designations： | 2Q5 |
| Species： | Homo sapiens, human |
| Depositors： | F Kao |
| Origin： | lymphoblast cell line GM03714 (46, XX) |
| Applications： | Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes. |
| Vector： | Tissue: lymphoblast cell line GM03714 (46, XX) Chromosome: 2q21-q22 DNA: genomic Digest: Complete Insert size range: 50-600 bp |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes. Approximately 50% of the clones contain repetitive sequences. Of 37 single copy clones tested, 26 (70%) hybridized to chromosome 2. Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19. |
| References： | Kao FT, et al. Complete set of eleven region-specific microdissection libraries for human chromosome 2. Somatic Cell Mol. Genet. 22: 57-66, 1996. PubMed: 8643994 |