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| 产品名称： | Galpha Q209L HA alpha q-Q209L (mouse) |
|---|---|
| 商品货号： | TS150357 |
| Designations： | Galpha Q209L HA alpha q-Q209L (mouse) |
| GenBank Number： | M55412 |
| Species： | Mus musculus, mouse |
| Depositors： | HR Bourne, P Herzmark |
| Applications： | in another host, produces protein guanine nucleotide binding protein (G protein), q polypeptide heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Gene product: guanine nucleotide binding protein (G protein), q polypeptide( heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q) Gnaq Target Gene: guanine nucleotide binding protein (G protein), q polypeptide |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2; HindIII--5.2; PstI--5.2; XbaI--5.2; XhoI--5.2. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.75; BglII--0.93; EcoRI--1.02; NdeI--0.48; NotI--0.04; PstI--0.08, 1.15; PvuII--1.15; SacI--0.22; SalI--0.26, 0.97; SmaI--0.02; XbaI--0.94; XhoI--1.02. Deposited with the understanding that the DNAs not be used in human experiments. The protein produced is the Q209L mutation (leucine replacing glutamine at codon 209) with a hemagglutinin tag. Nucleotides 93-95 and 687 of the GenBank sequence have been altered to add restriction sites for SalI and BamHI respectively. This does not alter the amino acid sequence. Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence. Restriction site(s) at the 3 end of the insert for linearizing the construct: NsiI. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Wedegaertner PB, et al. Palmitoylation is required for signaling functions and membrane attachment of Gq-alpha and Gs-alpha. J. Biol. Chem. 268: 25001-25008, 1993. PubMed: 8227063 Paul Herzmark, personal communication |