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| 产品名称： | pFN476 |
|---|---|
| 商品货号： | TS150400 |
| Designations： | pFN476 |
| Depositors： | P Sankar |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.320 Vector: pFN476 (plasmid) Promoters: Promoter lac Construction: pGEM3Z, pKA101-6 Marker(s):ampR Construct size (kb): 4.320 Features: insert detection: lacZ marker(s): ampR other: par promoter: lac promoter for expression: T7 promoter for in vitro transcription: SP6 replicon: pSC101 replicon: rep (pSC101) MCS: pGEM-3Z; HindIII...EcoRI restriction site: BamHI coding sequence: tnpR |
| Applications： | expression vector T7 promoter vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1. Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects. Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell. Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts. T7 RNA polymerase can also be provided by infection of F hosts with M13mGP1-2 (ATCC 40303). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sankar P, et al. Expression analysis of cloned chromosomal segments of Escherichia coli. J. Bacteriol. 175: 5145-5152, 1993. PubMed: 8349554 |
| Shipped： | frozen |