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| 产品名称： | pUN70 Y302, pNN336 |
|---|---|
| 商品货号： | TS150444 |
| Designations： | pUN70 Y302, pNN336 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.6999998092651370 Vector: pUN70 (phagemid) Promoters: Promoter lac Construction: pUC18 Marker(s):URA3,ampR Construct size (kb): 5.699999809265137 Features: insert detection: lacZ marker(s): URA3 marker(s): ampR promoter: lac replicon: ARS1 replicon: M13+ replicon: pMB1 MCS: EcoRI...HindIII centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3. YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604 |
| Related Products： | component of:ATCC 87634 component of:ATCC 87637 |
| Shipped： | freeze-dried |