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| 产品名称： | Rat coronavirus |
|---|---|
| 商品货号： | TS150552 |
| Classification： | Nidovirales, Coronaviridae, Coronavirus,Group 2 |
| Deposited As： | Rat Coronavirus |
| Agent： | Rat coronavirus |
| Strain： | 8190 |
| Common Name： | Rat coronavirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Lungs of rat |
| Product Format： | frozen |
| Storage Conditions： | xa0Store at -70°C or colder |
| Comments： | Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents. VR-1410 and VR-635 are the same strain provided by different contributors. Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents. |
| Effect on Host： | Yes, in vitro effects: Causes large syncytia in L2 or PRK cells Yes, in vivo effects: respiratory distress and death in suckling rats |
| Recommended Host： | L2 cells (ATCC CCL-149) Recommended Host: xa0L2 (Percy) (ATCC® CCL-81x99) rat; mouse; L2 cells; primary rat kidney cells |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and rinse cell sheet with 1X DPBS. Remove 1X DPBS and then inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.01. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. |
| Name of Depositor： | DJ Gaertner |
| Special Collection： | ATCC |
| Source： | Lungs of rat |
| References： | Parker JC, et al. Rat coronavirus (RCV): a prevalent, naturally occurring pneumotropic virus of rats. Arch. Gesamte Virusforsch. 31: 293-302, 1970. PubMed: 4099196 |