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| 产品名称： | pUR18N |
|---|---|
| 商品货号： | TS150584 |
| Designations： | pUR18N |
| Depositors： | NC Barbet, AM Carr |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5000000000000000 Vector: pUR18N (plasmid) Promoters: Promoter lac Construction: pUR18 (ATCC 77297) Marker(s):ampR,ura4+ Construct size (kb): 5.5 Features: insert detection: lacZ marker(s): ampR, ura4 promoter: lac replicon: pMB1, ars1 |
| Applications： | expression vector shuttle vector vector for other uses vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions and facilitates the rescue in E. coli of plasmids where dimerization has occurred. The order of the major features in this plasmid is: ars1 - ura4 - SfiI/MCS/EcoRI/lacZ - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485 |
| Shipped： | freeze-dried |