| 产品名称: | pBYr5 |
|---|---|
| 商品货号: | TS150612 |
| Designations: | pBYr5 |
| Species: | Bean yellow mosaic virus |
| Depositors: | J Hammond, DJ Houser |
| Vector: | Construct size (kb): 3.450000047683716 |
| Insert: | DNA: cDNA Insert lengths(kb): 0.75 Gene product: genome, partial |
| Insert Size (kb): | 0.75 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | The insert has an internal HindIII site about 170 bp from the vector HindIII site. The insert also contains DdeI sites (2), TaqI sites (2), Sau3A1 sites (2) , and a RsaI site. Restriction digests of the clone give the following sizes (kb): PstI--2.7, 0.75; EcoRI--3.4; HindIII--3.3, 0.2. Also available from the ATCC are pBYr5aSP (ATCC 45075) and pBYr5bSP (ATCC 45076) which contain the 0.75 kb PstI fragment cloned in opposite orientations into pSP64. pBYr5 was constructed by random priming. |
| Classification: | Potyvirus |
| References: | John Hammond, personal communication |