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微信小章| 产品名称： | Human adenovirus 19 |
|---|---|
| 商品货号： | TS150629 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Deposited As： | Adenovirus type 19 |
| Agent： | Human adenovirus 19 |
| Strain： | AV-587 3911 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Comments： | Replicates very slowly. Agglutinates rat RBC. Identical to adenovirus type 10 in HAI but different in neutralization tests
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20: 613, 1963. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | KB cells (ATCC® CCL-17™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 6-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | SD Bell |
| Source： | Conjunctival scrapings from child with trachoma, Saudi Arabia, 1955 |
| Year of Origin： | 1955 |
| References： | Bell SD. . Am. J. Trop. Med. Hyg. 9: 523, 1960. Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 |