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微信小章| 产品名称： | HULEC-5a |
|---|---|
| 商品货号： | TS150686 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Cell Type： | microvascular endothelium |
| Product Format： | frozen |
| Morphology： | endothelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | newborn |
| Gender： | male |
| Applications： | Investigating the role of endothelium in infection by virus and bacteria, cancer research, tumor metastastis, endothelium function, cell trafficking, surface molecule interactions |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Microvascular endothelial cells isolated from human lungs were transfected with pSVT vector, xa0a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen. |
| Receptor Expression： | neonatal FcRn receptor (FcRn) (Goebl N, et al. Neonatal Fc Receptor Mediates Internalization of Fc in Transfected Human Endothelial Cells. Mol. Biol. Cell 19: 5490-5505, 2008. PubMed: 18843053) |
| Comments： | CRL-3244, HULEC-5a, has been shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human lung endothelial cells for many research studies. |
| Complete Growth Medium： | The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.xa0 Medium Renewal: Do not feed cells with growth medium between subcultures or cells become very tightly attached and will be difficult to disperse with trypsin-EDTA. |
| Cryopreservation： | Freeze Medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11, 12 D13S317: 13, 14 D16S539: xa09, 10 D5S818: 11, 13 D7S820: 8, 10 THO1: 6, 7 TPOX: 8, 11 vWA: 16, 17 |
| Name of Depositor： | Kathryn Kellar, Ph. D. |
| Year of Origin： | November 1990 |
| References： | Lawrence EC et al. Establishment of immortalized human lung microvascular endothelial cell lines. Chest 108(3) Supplement:126S, 1995. Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507 Mehta P, et al. Entry and intracellular replication of Mycobacterium tuberculosis in cultured human microvascular endothelial cells. Microb. Pathog. 41: 119-124, 2006. PubMed: 16860530 Goebl N, et al. Neonatal Fc Receptor Mediates Internalization of Fc in Transfected Human Endothelial Cells. Mol. Biol. Cell 19: 5490-5505, 2008. PubMed: 18843053 |