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| 产品名称： | EBOpLPP |
|---|---|
| 商品货号： | TS150738 |
| Designations： | EBOpLPP |
| Depositors： | RF Margolskee |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5719995498657200 Vector: EBOpLPP (plasmid) Promoters: Promoter SV40 early Construction: pcDV, pL, EBOpSV2neo Marker(s):ampR,hygR Construct size (kb): 10.57199954986572 Features: marker(s): ampR marker(s): hygR promoter: SV40 early replicon: EBV oriP replicon: pMB1 terminator: SV40 enhancer: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6. This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning. This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene). This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Canfield V, et al. Ouabain-resistant mutants of the rat Na,K-ATPase alpha2 isoform identified by using an episomal expression vector. Mol. Cell. Biol. 10: 1367-1372, 1990. PubMed: 2157137 Spickofsky N, et al. Procedures for constructing cDNA expression libraries in Epstein-Barr virus shuttle vectors capable of stable episomal replication. DNA Protein Eng. Tech. 2: 14-18, 1990. |
| Shipped： | freeze-dried |