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微信小章| 产品名称： | Vahlkampfia lobospinosa (Craig) Craig |
|---|---|
| 商品货号： | TS150787 |
| Application： | Restriction endonuclease analysis of mitochondrial DNA |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | cattle feces, Shelby County, TN, 1969 |
| Product Format： | dried |
| Type Strain： | no |
| Comments： | Circular ribosomal RNA genes biochemical identification Restriction endonuclease analysis of mitochondrial DNA Identification and phylogenetic relationships Promitosis use of plastic ampoules for freeze preservation |
| Medium： | ATCC® Medium 711: PYB |
| Growth Conditions： | Temperature: 35.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Name of Depositor： | WE Wilhelm, ER Fizer |
| Year of Origin： | 1969 |
| References： | Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 Milligan SM, Band RN. Restriction endonuclease analysis of mitochondrial DNA as an aid in the taxonomy of Naegleria and Vahlkampfia. J. Protozool. 35: 198-204, 1988. PubMed: 2840491 Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 Wilhelm WE, Anderson JH. Vahlkampfia lobospinosa (Craig. 1912) Craig. 1913: rediscovery of a coprozoic ameba. J. Parasitol. 57: 1378-1379, 1971. PubMed: 5157177 Brown S, De Jonckheere JF. Identification and phylogenetic relationships of Vahlkampfia spp. (free-living amoebae) by riboprinting. FEMS Microbiol. Lett. 115: 241-246, 1994. Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 Fizer ER, Wilhelm WE. Promitosis and rod occurrence in the limax ameba Vahlkampfia lobospinosa. J. Protozool. 25: 34-38, 1978. PubMed: 660570 |
| Cross References： | Nucleotide (GenBank) : M98052 Vahlkampfia lobospinosa 16S ribosoma RNA-like gene. |