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| 产品名称： | cMCOB19 RW-203 |
|---|---|
| 商品货号： | TS150815 |
| Designations： | cMCOB19 RW-203 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 28.0 |
| Insert： | DNA: genomic Insert lengths(kb): 20.0 Gene product: DNA Segment, single copy D19S20 |
| Insert Size (kb)： | 20.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HpaI--1.8, 1.2, 0.9 + smaller; HindIII--16.9, 3.6, 3.2, 2.2, 1.9; EcoRI--19.6, 7.5; XbaI--28; AccI--12.3, 7.2, 6.3, 4.4, 1.6. Depositor has verified that our preparation detects the described RFLP. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs observed under normal stringency (0.1X SSC). |
| References： | Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220 Yusuke Nakamura, personal communication |