微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBAD28 |
|---|---|
| 商品货号： | TS150892 |
| Designations： | pBAD28 |
| Depositors： | L Guzman, J Beckwith |
| Biosafety Level： | 1 |
| Vector Information： | Vector Information: Vector type: phagemid Vector size (kb): 5.801 Markers: ampR, cmlR, araC Polylinker sites: NheI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII Construction: pBAD18, pACYC184 Features: marker(s): ampR |
| Applications： | expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--3.1, 1.9, 1.3; BamHI--6.1; PstI--5.0;1.2. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. The following primers can be used for sequencing of cloned inserts: 5 primer (27 - 8 bp upstream of the NheI site) 5-CTGTTTCTCCATACCCGTT-3; and one of two 3 primers: 3 primer 1 (2 - 19 bp downstream of the HindIII site) 5-CTCATCCGCCAAAACAG-3; 3 primer 2 (17 - 33 bp downstream of the HindIII site) 5-GGCTGAAAATCTTCTCT-3. Plasmid copy number is low due to the p15A replicon. This vector can be used when reduced gene expression is desirable. Plasmid is compatible with pBR-derived plasmids and may be used for coexpression of cloned inserts. |
| Media： | ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of E. coli DH5-alpha; containing the plasmid. |