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微信小章| 产品名称： | pT7+117/M/D |
|---|---|
| 商品货号： | TS150954 |
| Designations： | pT7+117/M/D |
| Species： | tobacco ringspot virus |
| Depositors： | G Bruening, PA Feldstein |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7200000286102295 Gene product: RNA, satellite |
| Insert Size (kb)： | 0.720 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.0, 0.82; EcoRI--4.9; PstI--4.9; RNA transcripts can be generated from the vector T7 promoter following linearization with BamHI. Transcripts undergo autolytic processing. Contains the circularly permuted dimeric tobacco ringspot virus satellite RNA sequence of the pBB1 derivative plasmid pSP651. RNA transcripts contain 723 nt of STobRV RNA sequence (117 nt of promoter-proximal, 247 nt of promoter-distal, and 359 nt of monomeric STobRV RNA) preceded by 21 nt derived from the vector. |
| Classification： | Nepovirus |
| References： | Buzayan JM, et al. Autolytic processing of a phosphorothioate diester bond. Nucleic Acids Res. 16: 4009-4023, 1988. PubMed: 2453843 Elena SF, et al. Phylogeny of viroids, viroidlike satellite RNAs, and the viroidlike domain of hepatitis delta virus RNA. Proc. Natl. Acad. Sci. USA 88: 5631-5634, 1991. PubMed: 1712103 Van Tol H, et al. Evidence for spontaneous circle formation in the replication of the satellite RNA of tobacco ringspot virus. Virology 180: 23-30, 1991. PubMed: 1984650 |